Introducing of the new methods of treatment of MM patients is connected with necessity of searching for more sophisticated methods of MRD evaluation and earlier diagnosis and treatment of relapse. The most often used methods are the fenotyping with flow cytometry and different types of qualitative and quantitative PCR. The merits and drawbacks of different method MRD evaluation are described.

Introducing of the new methods of treatment of MM patients is connected with necessity of searching for more sophisticated methods of MRD evaluation and earlier diagnosis and treatment of relapse. The most often used methods are the fenotyping with flow cytometry and different types of qualitative and quantitative PCR. The merits and drawbacks of different method MRD evaluation are described.

The primary disadvantage of cord blood as a source of cells for transplantation , the number of cells is small , usually less than 1 billion times, allowing operation only in children under 30 kg , while the majority of recipients has a larger weight. Hence the idea of simultaneous use of several units of cord blood , which together are designed to provide a sufficient number of cells for transplantation to an adult recipient. The bottom line is that the immune cells in cord blood , as young cells should not be able to cross- reactions against each other.