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Human skin is an organ rich in stem cells cultured in vitro form a so-called . holoklony . These cells express high levels of ? 1 - and ? 6 - integrin , p63 proteins , Delta1 , and ? - Catenin . Early keratinocytes are of much interest in research of both basic and clinical research , due to the possibility of their potential use in the treatment of trophic ulcers and burns . Although propagated from small biopsies ex vivo keratinocytes are successfully used in the clinic , our knowledge about the mechanisms regulating self-renewal and differentiation of skin stem cells is very limited. The aim of our research is to obtain a pure population of stem cells and epidermal proliferation as efficient for clinical use . We found that c -kit receptor is very useful in the purification of the fraction of keratinocytes with melanocytes in primary culture . We have also started early attempts keratinocytes phenotyping using selected antibody ( against CXCR1 , CD44H , c -met , ? 3 -, 5 - ,6- integrins , FAS ) to identify surface markers that could be used to isolate the stem cells of the skin. Serving as a marker of the early isolation of the forming epidermal cells in vitro , we used antibodies holoklony ? Simultaneously 1 - integrin cell staining with rhodamine 123 We observed that cells with rhodamine staining least 123 and at the same time exhibiting the highest expression of ? 1 - integrin are highly enriched in cells in culture so forming . holokony . Using this sub-population of cells then tested the effect of selected growth factors and cytokines on the proliferation and differentiation . We have tried to correlate the data from both in vitro culture with the activation of selected signal transduction pathways regulating cell proliferation and survival .
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Andrzej Łukaszyk - przewodniczący, Zofia Bielańska-Osuchowska, Szczepan Biliński, Mieczysław Chorąży, Aleksander Koj, Włodzimierz Korochoda, Leszek Kuźnicki, Aleksandra Stojałowska, Lech Wojtczak

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