The ImageStream technology was developed as a novel tool for multiparameter cell ana- lysis. The system fully integrates the features of flow cytometry and fluorescent microscopy com- bined with a modern system of image analysis. Similarly to flow cytometry, the ImageStream allows analyzing of a large number of cells according to their fluorescent features and provides statistical analysis of these objects. Additionally, allows detailed morphometric analysis of cells based on their images collected during acquisition as well as correlation of various morphometric and photometric features of the cells. Simply, the ImageStream system is a flow cytometer collecting images of cells during their acquisition that allows their further multiparameter analysis. The innovative features of the instrument create multitude possible applications of the system, especially for experiments where flow cytometry does not provide enough information about analyzed cells. The aim of this article is to introduce brief information about the system to scientists working with various systems of image analysis or flow cytometry. The article summarizes basic information about the features of Image- Stream system as well as its possible applications.